A single cell atlas of the human liver tumor microenvironment.
IF: 13.068
Cited by: 62


Malignant cell growth is fueled by interactions between tumor cells and the stromal cells composing the tumor microenvironment. The human liver is a major site of tumors and metastases, but molecular identities and intercellular interactions of different cell types have not been resolved in these pathologies. Here, we apply single cell RNA-sequencing and spatial analysis of malignant and adjacent non-malignant liver tissues from five patients with cholangiocarcinoma or liver metastases. We find that stromal cells exhibit recurring, patient-independent expression programs, and reconstruct a ligand-receptor map that highlights recurring tumor-stroma interactions. By combining transcriptomics of laser-capture microdissected regions, we reconstruct a zonation atlas of hepatocytes in the non-malignant sites and characterize the spatial distribution of each cell type across the tumor microenvironment. Our analysis provides a resource for understanding human liver malignancies and may expose potential points of interventions.


Spatial Transcriptomics
human cell atlas
liver cancer
single cell RNAseq
spatial transcriptomics
tumor-stroma interactions

MeSH terms

Anatomy, Artistic
Atlases as Topic
Endothelial Cells
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Gene Regulatory Networks
Liver Neoplasms
Single-Cell Analysis
Tumor Microenvironment


Massalha, Hassan
Bahar Halpern, Keren
Abu-Gazala, Samir
Jana, Tamar
Massasa, Efi E
Moor, Andreas E
Buchauer, Lisa
Rozenberg, Milena
Pikarsky, Eli
Amit, Ido
Zamir, Gideon
Itzkovitz, Shalev

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