ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.
IF: 4.996
Cited by: 7


Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.


Spatial Genomics

MeSH terms

DNA Probes
Genome, Human
In Situ Hybridization, Fluorescence
Time Factors
User-Computer Interface


Hu, Mengwei
Yang, Bing
Cheng, Yubao
Radda, Jonathan S D
Chen, Yanbo
Liu, Miao
Wang, Siyuan

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