ProbeDealer is a convenient tool for designing probes for highly multiplexed fluorescence in situ hybridization.

IF: 4.996

Cited by: 7

Abstract

Fluorescence in situ hybridization (FISH) is a powerful method to visualize the spatial positions of specific genomic loci and RNA species. Recent technological advances have leveraged FISH to visualize these features in a highly multiplexed manner. Notable examples include chromatin tracing, RNA multiplexed error-robust FISH (MERFISH), multiplexed imaging of nucleome architectures (MINA), and sequential single-molecule RNA FISH. However, one obstacle to the broad adoption of these methods is the complexity of the multiplexed FISH probe design. In this paper, we introduce an easy-to-use, versatile, and all-in-one application called ProbeDealer to design probes for a variety of multiplexed FISH techniques and their combinations. ProbeDealer offers a one-stop shop for multiplexed FISH design needs of the research community.

Keywords

Spatial Genomics

MERFISH

MeSH terms

Animals

DNA Probes

Genome, Human

Humans

In Situ Hybridization, Fluorescence

Mice

Time Factors

User-Computer Interface

Authors

Hu, Mengwei

Yang, Bing

Cheng, Yubao

Radda, Jonathan S D

Chen, Yanbo

Liu, Miao

Wang, Siyuan

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