Visualizing subcellular rearrangements in intact β cells using soft x-ray tomography.
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IF: 14.957
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Cited by: 11
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Abstract

Characterizing relationships between cell structures and functions requires mesoscale mapping of intact cells showing subcellular rearrangements following stimulation; however, current approaches are limited in this regard. Here, we report a unique application of soft x-ray tomography to generate three-dimensional reconstructions of whole pancreatic β cells at different time points following glucose-stimulated insulin secretion. Reconstructions following stimulation showed distinct insulin vesicle distribution patterns reflective of altered vesicle pool sizes as they travel through the secretory pathway. Our results show that glucose stimulation caused rapid changes in biochemical composition and/or density of insulin packing, increased mitochondrial volume, and closer proximity of insulin vesicles to mitochondria. Costimulation with exendin-4 (a glucagon-like peptide-1 receptor agonist) prolonged these effects and increased insulin packaging efficiency and vesicle maturation. This study provides unique perspectives on the coordinated structural reorganization and interactions of organelles that dictate cell responses.

Authors

White, Kate L
Singla, Jitin
Loconte, Valentina
Chen, Jian-Hua
Ekman, Axel
Sun, Liping
Zhang, Xianjun
Francis, John Paul
Li, Angdi
Lin, Wen
Tseng, Kaylee
McDermott, Gerry
Alber, Frank
Sali, Andrej
Larabell, Carolyn
Stevens, Raymond C

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