A sensitive mNeonGreen reporter system to measure transcriptional dynamics in Drosophila development.
IF: 6.548
Cited by: 4


The gene regulatory network governing anterior-posterior axis formation in Drosophila is a well-established paradigm to study transcription in developmental biology. The rapid temporal dynamics of gene expression during early stages of development, however, are difficult to track with standard techniques. We optimized the bright and fast-maturing fluorescent protein mNeonGreen as a real-time, quantitative reporter of enhancer expression. We derive enhancer activity from the reporter fluorescence dynamics with high spatial and temporal resolution, using a robust reconstruction algorithm. By comparing our results with data obtained with the established MS2-MCP system, we demonstrate the higher detection sensitivity of our reporter. We used the reporter to quantify the activity of variants of a simple synthetic enhancer, and observe increased activity upon reduction of enhancer-promoter distance or addition of binding sites for the pioneer transcription factor Zelda. Our reporter system constitutes a powerful tool to study spatio-temporal gene expression dynamics in live embryos.


Temporal Gene Expression

MeSH terms

Binding Sites
Body Patterning
Drosophila Proteins
Embryo, Nonmammalian
Fluorescent Dyes
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Genes, Reporter
Promoter Regions, Genetic
Transcription Factors


Ceolin, Stefano
Hanf, Monika
Bozek, Marta
Storti, Andrea Ennio
Gompel, Nicolas
Unnerstall, Ulrich
Jung, Christophe
Gaul, Ulrike

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