Whole-Mount Immunofluorescence Staining of Early Mouse Embryos.
Abstract
Immunofluorescence staining enables the visualization of protein expression at a cellular or even sub-nuclear level. Whole-mount staining preserves the three-dimensional spatial information in biological samples allowing a comprehensive interpretation of expression domains. Here we describe the sample processing, protein detection using antibodies and confocal imaging of isolated preimplantation to early postimplantation mouse embryos up to Embryonic day 8.0 (E8.0).
Keywords
3D-FISH
Spatial Transcriptomics
SHIELD
Spatial Gene Expression
Embryo
Immunofluorescence
Immunohistochemistry
Postimplantation
Preimplantation
Whole-mount
MeSH terms
Animals
Embryo, Mammalian
Fluorescent Antibody Technique
Mice
Microscopy, Confocal
Microscopy, Fluorescence
Authors
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