Single-cell transcriptome analysis reveals cell lineage specification in temporal-spatial patterns in human cortical development.
IF: 14.957
Cited by: 67


Neurogenesis processes differ in different areas of the cortex in many species, including humans. Here, we performed single-cell transcriptome profiling of the four cortical lobes and pons during human embryonic and fetal development. We identified distinct subtypes of neural progenitor cells (NPCs) and their molecular signatures, including a group of previously unidentified transient NPCs. We specified the neurogenesis path and molecular regulations of the human deep-layer, upper-layer, and mature neurons. Neurons showed clear spatial and temporal distinctions, while glial cells of different origins showed development patterns similar to those of mice, and we captured the developmental trajectory of oligodendrocyte lineage cells until the human mid-fetal stage. Additionally, we verified region-specific characteristics of neurons in the cortex, including their distinct electrophysiological features. With systematic single-cell analysis, we decoded human neuronal development in temporal and spatial dimensions from GW7 to GW28, offering deeper insights into the molecular regulations underlying human neurogenesis and cortical development.



MeSH terms

Cell Differentiation
Cell Lineage
Cerebral Cortex
Gene Expression Profiling
Neural Stem Cells
Single-Cell Analysis


Fan, Xiaoying
Fu, Yuanyuan
Zhou, Xin
Sun, Le
Yang, Ming
Wang, Mengdi
Chen, Ruiguo
Wu, Qian
Yong, Jun
Dong, Ji
Wen, Lu
Qiao, Jie
Wang, Xiaoqun
Tang, Fuchou

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