Single cell transcriptomics identifies a signaling network coordinating endoderm and mesoderm diversification during foregut organogenesis.
IF: 17.694
Cited by: 101


Visceral organs, such as the lungs, stomach and liver, are derived from the fetal foregut through a series of inductive interactions between the definitive endoderm (DE) and the surrounding splanchnic mesoderm (SM). While DE patterning is fairly well studied, the paracrine signaling controlling SM regionalization and how this is coordinated with epithelial identity is obscure. Here, we use single cell transcriptomics to generate a high-resolution cell state map of the embryonic mouse foregut. This identifies a diversity of SM cell types that develop in close register with the organ-specific epithelium. We infer a spatiotemporal signaling network of endoderm-mesoderm interactions that orchestrate foregut organogenesis. We validate key predictions with mouse genetics, showing the importance of endoderm-derived signals in mesoderm patterning. Finally, leveraging these signaling interactions, we generate different SM subtypes from human pluripotent stem cells (hPSCs), which previously have been elusive. The single cell data can be explored at: .


Gene Expression

MeSH terms

Cell Lineage
Digestive System
Gene Expression Profiling
Gene Expression Regulation, Developmental
Gene Regulatory Networks
Mice, Inbred C57BL
Signal Transduction
Single-Cell Analysis
Transcription Factors


Han, Lu
Chaturvedi, Praneet
Kishimoto, Keishi
Koike, Hiroyuki
Nasr, Talia
Iwasawa, Kentaro
Giesbrecht, Kirsten
Witcher, Phillip C
Eicher, Alexandra
Haines, Lauren
Lee, Yarim
Shannon, John M
Morimoto, Mitsuru
Wells, James M
Takebe, Takanori
Zorn, Aaron M

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