A Dual Protein-mRNA Localization Screen Reveals Compartmentalized Translation and Widespread Co-translational RNA Targeting.
IF: 13.417
Cited by: 37


Local translation allows spatial control of gene expression. Here, we performed a dual protein-mRNA localization screen, using smFISH on 523 human cell lines expressing GFP-tagged genes. 32 mRNAs displayed specific cytoplasmic localizations with local translation at unexpected locations, including cytoplasmic protrusions, cell edges, endosomes, Golgi, the nuclear envelope, and centrosomes, the latter being cell-cycle-dependent. Automated classification of mRNA localization patterns revealed a high degree of intercellular heterogeneity. Surprisingly, mRNA localization frequently required ongoing translation, indicating widespread co-translational RNA targeting. Interestingly, while P-body accumulation was frequent (15 mRNAs), four mRNAs accumulated in foci that were distinct structures. These foci lacked the mature protein, but nascent polypeptide imaging showed that they were specialized translation factories. For β-catenin, foci formation was regulated by Wnt, relied on APC-dependent polysome aggregation, and led to nascent protein degradation. Thus, translation factories uniquely regulate nascent protein metabolism and create a fine granular compartmentalization of translation.


Spatial Gene Expression
RNA localization
RNA transport
co-translational targeting
local translation
translation factories

MeSH terms

Cell Line
Gene Expression Regulation
Protein Biosynthesis
Protein Transport
RNA, Messenger


Chouaib, Racha
Safieddine, Adham
Pichon, Xavier
Imbert, Arthur
Kwon, Oh Sung
Samacoits, Aubin
Traboulsi, Abdel-Meneem
Robert, Marie-Cécile
Tsanov, Nikolay
Coleno, Emeline
Poser, Ina
Zimmer, Christophe
Hyman, Anthony
Le Hir, Hervé
Zibara, Kazem
Peter, Marion
Mueller, Florian
Walter, Thomas
Bertrand, Edouard

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