Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection.
The relationship of SARS-CoV-2 lung infection and severity of pulmonary disease is not fully understood. We analyzed autopsy specimens from 24 patients who succumbed to SARS-CoV-2 infection using a combination of different RNA and protein analytical platforms to characterize inter- and intra- patient heterogeneity of pulmonary virus infection. There was a spectrum of high and low virus cases that was associated with duration of disease and activation of interferon pathway genes. Using a digital spatial profiling platform, the virus corresponded to distinct spatial expression of interferon response genes and immune checkpoint genes demonstrating the intra-pulmonary heterogeneity of SARS-CoV-2 infection.
1. Temporal and spatial heterogeneity of host response to SARS-CoV-2 pulmonary infection.
2. A single-cell and spatial atlas of autopsy tissues reveals pathology and cellular targets of SARS-CoV-2.
3. Profiling of lung SARS-CoV-2 and influenza virus infection dissects virus-specific host responses and gene signatures.
4. Temporal and Spatial Heterogeneity of Host Response to SARS-CoV-2 Pulmonary Infection.
5. COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets.
1. Spectrum of Viral Load and Host Response Seen in Autopsies of SARS-CoV-2 Infected Lungs
2. Single cell transcriptomics of human and mouse lung cancers reveals conserved myeloid populations across individuals and species
3. High-throughput tissue dissection and cell purification with digital cytometry [scRNA-Seq]
4. Single-cell landscape of bronchoalveolar immune cells in COVID-19 patients
5. SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues