Cryogenic OrbiSIMS Localizes Semi-Volatile Molecules in Biological Tissues.

IF: 16.823

Cited by: 17

Abstract

OrbiSIMS is a recently developed instrument for label-free imaging of chemicals with micron spatial resolution and high mass resolution. We report a cryogenic workflow for OrbiSIMS (Cryo-OrbiSIMS) that improves chemical detection of lipids and other biomolecules in tissues. Cryo-OrbiSIMS boosts ionization yield and decreases ion-beam induced fragmentation, greatly improving the detection of biomolecules such as triacylglycerides. It also increases chemical coverage to include molecules with intermediate or high vapor pressures, such as free fatty acids and semi-volatile organic compounds (SVOCs). We find that Cryo-OrbiSIMS reveals the hitherto unknown localization patterns of SVOCs with high spatial and chemical resolution in diverse plant, animal, and human tissues. We also show that Cryo-OrbiSIMS can be combined with genetic analysis to identify enzymes regulating SVOC metabolism. Cryo-OrbiSIMS is applicable to high resolution imaging of a wide variety of non-volatile and semi-volatile molecules across many areas of biomedicine.

Keywords

OrbiSIMS

SIMS

Cryo-OrbiSIMS

analytical methods

biological mass spectrometry imaging

lipids

semi-volatile molecules

MeSH terms

Cold Temperature

History, 15th Century

Mass Spectrometry

Volatile Organic Compounds

Authors

Newell, Clare L

Vorng, Jean-Luc

MacRae, James I

Gilmore, Ian S

Gould, Alex P

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