Specific labeling of synaptic schwann cells reveals unique cellular and molecular features.
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IF: 8.713
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Cited by: 21
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Abstract

Perisynaptic Schwann cells (PSCs) are specialized, non-myelinating, synaptic glia of the neuromuscular junction (NMJ), that participate in synapse development, function, maintenance, and repair. The study of PSCs has relied on an anatomy-based approach, as the identities of cell-specific PSC molecular markers have remained elusive. This limited approach has precluded our ability to isolate and genetically manipulate PSCs in a cell specific manner. We have identified neuron-glia antigen 2 (NG2) as a unique molecular marker of S100β+ PSCs in skeletal muscle. NG2 is expressed in Schwann cells already associated with the NMJ, indicating that it is a marker of differentiated PSCs. Using a newly generated transgenic mouse in which PSCs are specifically labeled, we show that PSCs have a unique molecular signature that includes genes known to play critical roles in PSCs and synapses. These findings will serve as a springboard for revealing drivers of PSC differentiation and function.

Keywords

NMJ
astrocytes
automated perimetrycytes
cell biology
mouse
neuroscience
perisynaptic schwann cells
synaptic glia
terminal schwann cells

MeSH terms

Animals
Antigens
Biomarkers
Mice
Mice, Transgenic
Muscle, Skeletal
Neuromuscular Junction
Proteoglycans
Schwann Cells

Authors

Castro, Ryan
Taetzsch, Thomas
Vaughan, Sydney K
Godbe, Kerilyn
Chappell, John
Settlage, Robert E
Valdez, Gregorio

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