Highly specific multiplexed RNA imaging in tissues with split-FISH.
Abstract
We present split-FISH, a multiplexed fluorescence in situ hybridization method that leverages a split-probe design to achieve enhanced specificity. Split-FISH reduces off-target background fluorescence, decreases false positives and enables accurate RNA profiling in uncleared tissues. We demonstrate the efficacy of split-FISH on various mouse tissues by quantifying the distribution and abundance of 317 genes in single cells and reveal diverse localization patterns for spatial regulation of the transcriptome in complex tissues.
Keywords
split-FISH
MeSH terms
Animals
Cells, Cultured
Humans
In Situ Hybridization, Fluorescence
Mice
RNA
Single-Cell Analysis
Transcriptome
Authors
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