The Role of Single-Cell Technology in the Study and Control of Infectious Diseases.
Cited by: 9
The advent of single-cell research in the recent decade has allowed biological studies at an unprecedented resolution and scale. In particular, single-cell analysis techniques such as Next-Generation Sequencing (NGS) and Fluorescence-Activated Cell Sorting (FACS) have helped show substantial links between cellular heterogeneity and infectious disease progression. The extensive characterization of genomic and phenotypic biomarkers, in addition to host-pathogen interactions at the single-cell level, has resulted in the discovery of previously unknown infection mechanisms as well as potential treatment options. In this article, we review the various single-cell technologies and their applications in the ongoing fight against infectious diseases, as well as discuss the potential opportunities for future development.
1. Fourth Generation of Next-Generation Sequencing Technologies: Promise and Consequences.
2. Applications of single-cell genomics and computational strategies to study common disease and population-level variation.
3. Single-cell RNA sequencing: Technical advancements and biological applications.
4. Challenges and emerging directions in single-cell analysis.
5. The Role of Single-Cell Technology in the Study and Control of Infectious Diseases.
1. Dissection of influenza infection in vivo by single-cell RNA-sequencing
2. Single-cell RNA-sequencing of Herpes simplex virus 1-infected cells identifies NRF2 activation as an antiviral program
3. Profiling 11,639 genes in PBMCs from 2 donors: U133 A 2.0 Custom CDF Version 9
4. Profiling 20,077 genes in 25 vaccine recipients: U133 Plus 2.0, Custom CDF Version 9
5. SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues