Confounding factors from inducible systems for spatiotemporal gene expression regulation.
Spatiotemporally regulated targeted gene manipulation is a common way to study the effect of gene variants on phenotypic traits, but the Cre/loxP and Tet-On/Tet-Off systems can affect whole-organism physiology and function due to off-target effects. We highlight some of these adverse effects, including whole-body endocrinology and disturbances in the gut microbiome and in mitochondrial and metabolic function.
Temporal Gene Expression
Gene Expression Regulation
Wüst, Rob C I
Houtkooper, Riekelt H
1. Dual Recombining-out System for Spatiotemporal Gene Expression in C. elegans.
2. Confounding factors from inducible systems for spatiotemporal gene expression regulation.
3. A practical approach to genetic inducible fate mapping: a visual guide to mark and track cells in vivo.
4. Advances in Genomics for Drug Development.