RNAscope™ Multiplex Detection in Zebrafish.
Abstract
The RNAscope methodology is a powerful tool to detect RNA expression patterns with high subcellular resolution and possibility for RNA-protein colocalization studies. Presented here is a two-day protocol for robust multiplex detection of up to three different RNAs in zebrafish whole-mount embryos using the RNAscope procedure. Application of the protocol offers the simultaneous detection of multiple RNAs with a high signal-to-noise ratio in an intact embryo.
Keywords
RNAscope
Gene Expression
FISH
High-resolution detection
In situ hybridization
RNA localization
WISH
Whole-mount embryo
Zebrafish
MeSH terms
Animals
Embryo, Nonmammalian
Gene Expression Regulation, Developmental
In Situ Hybridization
In Situ Hybridization, Fluorescence
Zebrafish
Authors
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