Changing Technologies of RNA Sequencing and Their Applications in Clinical Oncology.
IF: 5.738
Cited by: 63


RNA sequencing (RNAseq) is one of the most commonly used techniques in life sciences, and has been widely used in cancer research, drug development, and cancer diagnosis and prognosis. Driven by various biological and technical questions, the techniques of RNAseq have progressed rapidly from bulk RNAseq, laser-captured micro-dissected RNAseq, and single-cell RNAseq to digital spatial RNA profiling, spatial transcriptomics, and direct in situ sequencing. These different technologies have their unique strengths, weaknesses, and suitable applications in the field of clinical oncology. To guide cancer researchers to select the most appropriate RNAseq technique for their biological questions, we will discuss each of these technologies, technical features, and clinical applications in cancer. We will help cancer researchers to understand the key differences of these RNAseq technologies and their optimal applications.


Spatial Transcriptomics
RNA sequencing
bulk RNAseq
digital spatial profiling
fourth-generation RNAseq
next generation sequencing
single-cell RNAseq
spatial transcriptomics


Wang, Ye
Mashock, Michael
Tong, Zhuang
Mu, Xiaofeng
Chen, Hong
Zhou, Xin
Zhang, Hong
Zhao, Gexin
Liu, Bin
Li, Xinmin

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