Spatiotemporal single-cell analysis of gene expression in the mouse suprachiasmatic nucleus.

PMID:32066983

IF: 28.771

Cited by: 151

Datasets

Abstract

Mammalian circadian behaviors are orchestrated by the suprachiasmatic nucleus (SCN) in the ventral hypothalamus, but the number of SCN cell types and their functional roles remain unclear. We have used single-cell RNA-sequencing to identify the basic cell types in the mouse SCN and to characterize their circadian and light-induced gene expression patterns. We identified eight major cell types, with each type displaying a specific pattern of circadian gene expression. Five SCN neuronal subtypes, each with specific combinations of markers, differ in their spatial distribution, circadian rhythmicity and light responsiveness. Through a complete three-dimensional reconstruction of the mouse SCN at single-cell resolution, we obtained a standardized SCN atlas containing the spatial distribution of these subtypes and gene expression. Furthermore, we observed heterogeneous circadian gene expression between SCN neuron subtypes. Such a spatiotemporal pattern of gene regulation within the SCN may have an important function in the circadian pacemaker.

Keywords

smFISH

Spatial reconstruction

Seurat

Temporal Spatial Omics

Temporal Spatial Gene Expression

RNAscope

LCM-seq

MeSH terms

Animals

Atlases as Topic

Circadian Rhythm

Circadian Rhythm Signaling Peptides and Proteins

Gene Expression

Gene Expression Regulation

Genomics

Light

Male

Mice

Mice, Inbred C57BL

Neurons

Photic Stimulation

Single-Cell Analysis

Suprachiasmatic Nucleus

Authors

Wen, Shao'ang

Ma, Danyi

Zhao, Meng

Xie, Lucheng

Wu, Qingqin

Gou, Lingfeng

Zhu, Chuanzhen

Fan, Yuqi

Wang, Haifang

Yan, Jun

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