Spatiotemporal single-cell analysis of gene expression in the mouse suprachiasmatic nucleus.
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IF: 28.771
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Cited by: 151
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Abstract

Mammalian circadian behaviors are orchestrated by the suprachiasmatic nucleus (SCN) in the ventral hypothalamus, but the number of SCN cell types and their functional roles remain unclear. We have used single-cell RNA-sequencing to identify the basic cell types in the mouse SCN and to characterize their circadian and light-induced gene expression patterns. We identified eight major cell types, with each type displaying a specific pattern of circadian gene expression. Five SCN neuronal subtypes, each with specific combinations of markers, differ in their spatial distribution, circadian rhythmicity and light responsiveness. Through a complete three-dimensional reconstruction of the mouse SCN at single-cell resolution, we obtained a standardized SCN atlas containing the spatial distribution of these subtypes and gene expression. Furthermore, we observed heterogeneous circadian gene expression between SCN neuron subtypes. Such a spatiotemporal pattern of gene regulation within the SCN may have an important function in the circadian pacemaker.

Keywords

Temporal Spatial Gene Expression
LCM-seq
Seurat
Temporal Spatial Omics
RNAscope
smFISH
Spatial reconstruction

MeSH terms

Animals
Atlases as Topic
Circadian Rhythm
Circadian Rhythm Signaling Peptides and Proteins
Gene Expression
Gene Expression Regulation
Genomics
Light
Male
Mice
Mice, Inbred C57BL
Neurons
Photic Stimulation
Single-Cell Analysis
Suprachiasmatic Nucleus

Authors

Wen, Shao'ang
Ma, Danyi
Zhao, Meng
Xie, Lucheng
Wu, Qingqin
Gou, Lingfeng
Zhu, Chuanzhen
Fan, Yuqi
Wang, Haifang
Yan, Jun

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