Mass Cytometry Imaging for the Study of Human Diseases-Applications and Data Analysis Strategies.
PMID:31798587
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IF: 8.786
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Cited by: 115
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Abstract

High parameter imaging is an important tool in the life sciences for both discovery and healthcare applications. Imaging Mass Cytometry (IMC) and Multiplexed Ion Beam Imaging (MIBI) are two relatively recent technologies which enable clinical samples to be simultaneously analyzed for up to 40 parameters at subcellular resolution. Importantly, these "Mass Cytometry Imaging" (MCI) modalities are being rapidly adopted for studies of the immune system in both health and disease. In this review we discuss, first, the various applications of MCI to date. Second, due to the inherent challenge of analyzing high parameter spatial data, we discuss the various approaches that have been employed for the processing and analysis of data from MCI experiments.

Keywords

MIBI
Spatial Omics
IMC
analysis
cytometry
imaging cytometry
imaging mass cytometry (IMC)
mass cytometry (CyTOF)
multiplexed imaging
multiplexed ion beam imaging
single cell

MeSH terms

Data Analysis
Flow Cytometry
Humans
Image Cytometry

Authors

Baharlou, Heeva
Canete, Nicolas P
Cunningham, Anthony L
Harman, Andrew N
Patrick, Ellis

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