Comparative spatial lipidomics analysis reveals cellular lipid remodelling in different developmental zones of barley roots in response to salinity.
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IF: 7.947
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Cited by: 18
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Abstract

Salinity-induced metabolic, ionic, and transcript modifications in plants have routinely been studied using whole plant tissues, which do not provide information on spatial tissue responses. The aim of this study was to assess the changes in the lipid profiles in a spatial manner and to quantify the changes in the elemental composition in roots of seedlings of four barley cultivars before and after a short-term salt stress. We used a combination of liquid chromatography-tandem mass spectrometry, inductively coupled plasma mass spectrometry, matrix-assisted laser desorption/ionization mass spectrometry imaging, and reverse transcription - quantitative real time polymerase chain reaction platforms to examine the molecular signatures of lipids, ions, and transcripts in three anatomically different seminal root tissues before and after salt stress. We found significant changes to the levels of major lipid classes including a decrease in the levels of lysoglycerophospholipids, ceramides, and hexosylceramides and an increase in the levels of glycerophospholipids, hydroxylated ceramides, and hexosylceramides. Our results revealed that modifications to lipid and transcript profiles in plant roots in response to a short-term salt stress may involve recycling of major lipid species, such as phosphatidylcholine, via resynthesis from glycerophosphocholine.

Keywords

MALDI
Spatial Lipidomics
Spatial Metabolomics
barley
glycerophosphocholine
lipids
mass spectrometry imaging
metabolome
salinity
spatial metabolomics

MeSH terms

Ceramides
Chromatography, Liquid
Gene Expression Regulation, Plant
Glycerophospholipids
Hordeum
Ions
Lipid Metabolism
Lipidomics
Lipids
Metabolome
Metabolomics
Plant Roots
Salinity
Salt Stress
Salts
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry

Authors

Sarabia, Lenin D
Boughton, Berin A
Rupasinghe, Thusitha
Callahan, Damien L
Hill, Camilla B
Roessner, Ute

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