Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches.
IF: 6.208
Cited by: 7


Antistasin, which was originally discovered in the salivary glands of the Mexican leech Haementeria officinalis, was newly isolated from Helobdella austinensis. To confirm the temporal expression of antistasin during embryogenesis, we carried out semi-quantitative RT-PCR. Hau-antistasin1 was uniquely expressed at stage 4 of the cleavage and was strongly expressed in the late stages of organogenesis, as were other antistasin members. In order to confirm the spatial expression of antistasin, we performed fluorescence in situ hybridization in the late stages of organogenesis. The expression of each antistasin in the proboscis showed a similar pattern and varied in expression in the body. In addition, the spatial expression of antistasin orthologs in different leeches showed the possibility of different function across leech species. Hau-antistasin1 was expressed in the same region as hedgehog, which is a known mediator of signal transduction pathway. Hau-antistasin1 is probably a downstream target of Hedgehog signaling, involved in segment polarity signal pathway.


Temporal Spatial Gene Expression
hedgehog signaling
in situ hybridization

MeSH terms

Gene Expression Regulation, Developmental
Hedgehog Proteins
Invertebrate Hormones
Signal Transduction


Kwak, Hee-Jin
Park, Jeong-Su
Medina Jiménez, Brenda Irene
Park, Soon Cheol
Cho, Sung-Jin

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