Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification.
IF: 16.823
Cited by: 133


Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.


hybridization chain reaction
signal amplification

MeSH terms

Cell Line
Infrared Rays
Nucleic Acid Amplification Techniques
Nucleic Acid Hybridization
Spectrometry, Fluorescence


Chu, Hongqian
Zhao, Jian
Mi, Yongsheng
Zhao, Yuliang
Li, Lele

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