Near-Infrared Light-Initiated Hybridization Chain Reaction for Spatially and Temporally Resolved Signal Amplification.

IF: 16.823

Cited by: 133

Abstract

Precise control over signal amplification provides unparalleled opportunities for diverse applications. However, spatiotemporally controlled amplification has not been realized because of the lack of a design methodology. The aim of this study was thus to develop a conceptual approach for remote control over signal amplification at a chosen time and site in living cells. This system was constructed by re-engineering the functional units of the hybridization chain reaction (HCR) and combination with upconversion photochemistry, thus resulting in an activatable HCR with the high spatial and temporal precision of near-infrared (NIR) light. As a proof of concept, we demonstrate the spatially and temporally resolved amplified imaging of messenger RNA (mRNA) with ultrahigh sensitivity in vitro and in vivo. Furthermore, by using a system targeting subcellular sites we have developed a new technique for NIR-initiated amplified imaging of mRNA exclusively within a specific organelle.

Keywords

RNA

bioimaging

fluorescence

hybridization chain reaction

signal amplification

MeSH terms

Cell Line

Humans

Infrared Rays

Nucleic Acid Amplification Techniques

Nucleic Acid Hybridization

Spectrometry, Fluorescence

Authors

Chu, Hongqian

Zhao, Jian

Mi, Yongsheng

Zhao, Yuliang

Li, Lele

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