Barcoded DNA nanostructures for the multiplexed profiling of subcellular protein distribution.
IF: 29.234
Cited by: 47


Massively parallel DNA sequencing is established, yet high-throughput protein profiling remains challenging. Here, we report a barcoding approach that leverages the combinatorial sequence content and the configurational programmability of DNA nanostructures for high-throughput multiplexed profiling of the subcellular expression and distribution of proteins in whole cells. The barcodes are formed by in situ hybridization of tetrahedral DNA nanostructures and short DNA sequences conjugated with protein-targeting antibodies, and by nanostructure-assisted ligation (either enzymatic or chemical) of the nanostructures and exogenous DNA sequences bound to nanoparticles of different sizes (which cause these localization sequences to differentially distribute across subcellular compartments). Compared with linear DNA barcoding, the nanostructured barcodes enhance the signal by more than 100-fold. By implementing the barcoding approach on a microfluidic device for the analysis of rare patient samples, we show that molecular subtypes of breast cancer can be accurately classified and that subcellular spatial markers of disease aggressiveness can be identified.


Gene Expression

MeSH terms

Base Sequence
Cell Line, Tumor
DNA Barcoding, Taxonomic
Gene Expression Profiling
Lab-On-A-Chip Devices
Staining and Labeling


Sundah, Noah R
Ho, Nicholas R Y
Lim, Geok Soon
Natalia, Auginia
Ding, Xianguang
Liu, Yu
Seet, Ju Ee
Chan, Ching Wan
Loh, Tze Ping
Shao, Huilin

Recommend literature

Similar data