A 3D molecular atlas of the chick embryonic heart.
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IF: 3.148
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Cited by: 10
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Abstract

We present a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq of 13 micro-dissected regions reveals regionalised expression of 15,570 genes. Of these, 132 were studied by in situ hybridisation and a subset (38 genes) was mapped by Optical Projection Tomography or serial sectioning to build a detailed 3-dimensional atlas of expression. We display this with a novel interactive 3-D viewer and as stacks of sections, revealing the boundaries of expression domains and regions of overlap. Analysis of the expression domains also defines some sub-regions distinct from those normally recognised by anatomical criteria at this stage of development, such as a previously undescribed subdivision of the atria into two orthogonal sets of domains (dorsoventral and left-right). We also include a detailed comparison of expression in the chick with the mouse and other species.

Keywords

Gene Expression
Tomo-seq
Anatomic
Cardiac
Cardiovascular
Chick embryo
Molecular anatomy
RNAseq
Transcriptome

MeSH terms

Anatomy, Artistic
Animals
Atlases as Topic
Chick Embryo
Chickens
Gene Expression
Gene Expression Regulation, Developmental
Heart
Imaging, Three-Dimensional
In Situ Hybridization

Authors

Anderson, Claire
Hill, Bill
Lu, Hui-Chun
Moverley, Adam
Yang, Youwen
Oliveira, Nidia M M
Baldock, Richard A
Stern, Claudio D

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