A 3D molecular atlas of the chick embryonic heart.
PMID:31283921
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IF: 3.148
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Cited by: 12
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Abstract

We present a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq of 13 micro-dissected regions reveals regionalised expression of 15,570 genes. Of these, 132 were studied by in situ hybridisation and a subset (38 genes) was mapped by Optical Projection Tomography or serial sectioning to build a detailed 3-dimensional atlas of expression. We display this with a novel interactive 3-D viewer and as stacks of sections, revealing the boundaries of expression domains and regions of overlap. Analysis of the expression domains also defines some sub-regions distinct from those normally recognised by anatomical criteria at this stage of development, such as a previously undescribed subdivision of the atria into two orthogonal sets of domains (dorsoventral and left-right). We also include a detailed comparison of expression in the chick with the mouse and other species.

Keywords

Tomo-seq
Anatomic
Gene Expression
Cardiac
Cardiovascular
Chick embryo
Molecular anatomy
RNAseq
Transcriptome

MeSH terms

Anatomy, Artistic
Animals
Atlases as Topic
Chick Embryo
Chickens
Gene Expression
Gene Expression Regulation, Developmental
Heart
Imaging, Three-Dimensional
In Situ Hybridization

Authors

Anderson, Claire
Hill, Bill
Lu, Hui-Chun
Moverley, Adam
Yang, Youwen
Oliveira, Nidia M M
Baldock, Richard A
Stern, Claudio D

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