A 3D molecular atlas of the chick embryonic heart.

IF: 3.148

Cited by: 10

Abstract

We present a detailed analysis of gene expression in the 2-day (HH12) embryonic chick heart. RNA-seq of 13 micro-dissected regions reveals regionalised expression of 15,570 genes. Of these, 132 were studied by in situ hybridisation and a subset (38 genes) was mapped by Optical Projection Tomography or serial sectioning to build a detailed 3-dimensional atlas of expression. We display this with a novel interactive 3-D viewer and as stacks of sections, revealing the boundaries of expression domains and regions of overlap. Analysis of the expression domains also defines some sub-regions distinct from those normally recognised by anatomical criteria at this stage of development, such as a previously undescribed subdivision of the atria into two orthogonal sets of domains (dorsoventral and left-right). We also include a detailed comparison of expression in the chick with the mouse and other species.

Keywords

Gene Expression

Tomo-seq

Anatomic

Cardiac

Cardiovascular

Chick embryo

Molecular anatomy

RNAseq

Transcriptome

MeSH terms

Anatomy, Artistic

Animals

Atlases as Topic

Chick Embryo

Chickens

Gene Expression

Gene Expression Regulation, Developmental

Heart

Imaging, Three-Dimensional

In Situ Hybridization

Authors

Anderson, Claire

Hill, Bill

Lu, Hui-Chun

Moverley, Adam

Yang, Youwen

Oliveira, Nidia M M

Baldock, Richard A

Stern, Claudio D

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