In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes.
In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.
In situ hybridization
Gene Expression Regulation, Developmental
In Situ Hybridization
Feng, Jian Q
1. General introduction to in situ hybridization protocol using nonradioactively labeled probes to detect mRNAs on tissue sections.
2. RNA in situ hybridization in Arabidopsis.
3. In situ hybridization.
4. Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci.
5. In situ hybridization (both radioactive and nonradioactive) and spatiotemporal gene expression analysis.