In Situ Hybridization on Mouse Paraffin Sections Using DIG-Labeled RNA Probes.
Abstract
In situ hybridization is a commonly used technique using an antisense RNA probe to localize a specific RNA sequence on histological sections. This approach can visualize the expression pattern of a gene of interest in a portion of tissues. Here, we detail an optimized method for performing in situ hybridization on mouse paraffin sections using digoxigenin (DIG)-labeled RNA probes.
Keywords
Gene Expression
Digoxigenin labeling
Gene expression
In situ hybridization
Paraffin section
RNA probe
RNase-free
mRNA
MeSH terms
Animals
Digoxigenin
Embryo, Mammalian
Gene Expression Regulation, Developmental
In Situ Hybridization
Mice
Paraffin Embedding
RNA Probes
RNA, Messenger
Authors
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