Multispectral tracing in densely labeled mouse brain with nTracer.

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Cited by: 13

Abstract

This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. Supplementary data are available at Bioinformatics online.

MeSH terms

Animals

Brain

Documentation

Image Processing, Computer-Assisted

Mice

Neurons

Software

Authors

Roossien, Douglas H

Sadis, Benjamin V

Yan, Yan

Webb, John M

Min, Lia Y

Dizaji, Aslan S

Bogart, Luke J

Mazuski, Cristina

Huth, Robert S

Stecher, Johanna S

Akula, Sriakhila

Shen, Fred

Li, Ye

Xiao, Tingxin

Vandenbrink, Madeleine

Lichtman, Jeff W

Hensch, Takao K

Herzog, Erik D

Cai, Dawen

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