Detection of miRNAs by Tissue Printing and Dot Blot Hybridization.
Abstract
Tissue printing and dot blot are simple techniques to detect miRNA expression and localization, allowing a better understanding of the function of a miRNA. In this work, we describe a tissue printing and a dot blot hybridization protocol for miRNA detection and localization in plant tissues, which opens the possibility of analyzing spatiotemporal expression patterns of miRNAs.
Keywords
Temporal Gene Expression
Dot blot
Hybridization
Nylon membrane
Tissue printing
miRNA detection
microRNAs
MeSH terms
Immunoblotting
In Situ Hybridization
MicroRNAs
Plants
RNA, Plant
Spatio-Temporal Analysis
Authors
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