Dynamic Organellar Maps for Spatial Proteomics.
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Eukaryotic cells are highly compartmentalized and protein subcellular localization critically influences protein function. Identification of the subcellular localizations of proteins and their translocation events upon perturbation has mostly been confined to targeted studies or laborious microscopy-based methods. Here we describe a systematic mass spectrometry-based method for spatial proteomics. The approach uses simple fractionation profiling and has two applications: Firstly it can be used to infer subcellular protein localization on a proteome-wide scale, resulting in a protein map of the cell. Secondly, the method permits identification of changes in protein localization, by comparing maps made under different conditions, as a tool for unbiased systems cell biology. © 2018 by John Wiley & Sons, Inc.


Spatial Proteomics
mass spectrometry
organelle proteomics
protein dynamics
spatial proteomics
subcellular localization
systems biology

MeSH terms

Intracellular Space
Mass Spectrometry
Subcellular Fractions


Itzhak, Daniel N
Schessner, Julia P
Borner, Georg H H

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