ClampFISH detects individual nucleic acid molecules using click chemistry-based amplification.
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IF: 68.164
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Cited by: 91
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Abstract

Methods for detecting single nucleic acids in cell and tissues, such as fluorescence in situ hybridization (FISH), are limited by relatively low signal intensity and nonspecific probe binding. Here we present click-amplifying FISH (clampFISH), a method for fluorescence detection of nucleic acids that achieves high specificity and high-gain (>400-fold) signal amplification. ClampFISH probes form a 'C' configuration upon hybridization to the sequence of interest in a double helical manner. The ends of the probes are ligated together using bio-orthogonal click chemistry, effectively locking the probes around the target. Iterative rounds of hybridization and click amplify the fluorescence intensity. We show that clampFISH enables the detection of RNA species with low-magnification microscopy and in RNA-based flow cytometry. Additionally, we show that the modular design of clampFISH probes allows multiplexing of RNA and DNA detection, that the locking mechanism prevents probe detachment in expansion microscopy, and that clampFISH can be applied in tissue samples.

Keywords

ClampFish

Authors

Rouhanifard, Sara H
Mellis, Ian A
Dunagin, Margaret
Bayatpour, Sareh
Jiang, Connie L
Dardani, Ian
Symmons, Orsolya
Emert, Benjamin
Torre, Eduardo
Cote, Allison
Sullivan, Alessandra
Stamatoyannopoulos, John A
Raj, Arjun

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