Visualizing phosphatidylcholine via mass spectrometry imaging: relevance to human health.

IF: 4.250

Cited by: 5

Abstract

Mass spectrometry imaging (MSI) techniques are nowadays widely used to obtain spatially resolved metabolite information from biological tissues. Since (phospho)lipids occur in all animal tissues and are very sensitively detectable, they are often in the focus of such studies. This particularly applies for phosphatidylcholines (PC) which are very sensitively detectable as positive ions due to the permanent positive charge of their choline headgroup. Areas covered: After a short introduction of lipid species occurring in biological systems and approaches normally used to obtain spatially resolved mass spectra (with the focus on matrix-assisted laser desorption/ionization coupled to time-of-flight (MALDI-TOF) MSI) a survey will be given which diseases have so far been characterized by changes of the PC composition. Expert commentary: Since PC species are very sensitively detectable by MS, sensitivity is not a major issue. However, spatial resolution is still limited and cellular dimensions can be hardly resolved by MALDI-TOF MSI, which is a critical point of the available approaches. Due to lacks of reproducibility and standardization further development is required.

Keywords

MALDI-MSI

SIMS

MALDI

MALDI-TOF MSI

PC-related diseases

Phosphatidylcholines (PC)

MeSH terms

Animals

Diagnostic Imaging

Humans

Mass Spectrometry

Phosphatidylcholines

Authors

Leopold, Jenny

Popkova, Yulia

Engel, Kathrin M

Schiller, Jürgen

Recommend literature

1. Nitrogen and Sulfur Co-doped Carbon-Dot-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Imaging for Profiling Bisphenol S Distribution in Mouse Tissues.

2. Mass Spectrometry Imaging (MSI) for Plant Metabolomics.

3. Batch Effects in MALDI Mass Spectrometry Imaging.

4. Spatially resolved metabolic distribution for unraveling the physiological change and responses in tomato fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI).

5. Metabolomic insights into the effects of thyroid hormone on Rana [Lithobates] catesbeiana metamorphosis using whole-body Matrix Assisted Laser Desorption/Ionization-Mass Spectrometry Imaging (MALDI-MSI).