Transcriptional Convergence of Oligodendrocyte Lineage Progenitors during Development.

IF: 13.417

Cited by: 118

Abstract

Pdgfra+ oligodendrocyte precursor cells (OPCs) arise in distinct specification waves during embryogenesis in the central nervous system (CNS). It is unclear whether there is a correlation between these waves and different oligodendrocyte (OL) states at adult stages. Here, we present bulk and single-cell transcriptomics resources providing insights on how transitions between these states occur. We found that post-natal OPCs from brain and spinal cord present similar transcriptional signatures. Moreover, post-natal OPC progeny of E13.5 Pdgfra+ cells present electrophysiological and transcriptional profiles similar to OPCs derived from subsequent specification waves, indicating that Pdgfra+ pre-OPCs rewire their transcriptional network during development. Single-cell RNA-seq and lineage tracing indicates that a subset of E13.5 Pdgfra+ cells originates cells of the pericyte lineage. Thus, our results indicate that embryonic Pdgfra+ cells in the CNS give rise to distinct post-natal cell lineages, including OPCs with convergent transcriptional profiles in different CNS regions.

Keywords

Omics

myelin

neural development

neural progenitors

oligodendrocyte

oligodendrocyte precursor cell

pericyte

platelet-derived growth factor receptor alpha

single-cell RNA-seq

transcription factors

transcriptomics

MeSH terms

Animals

Cell Differentiation

Cell Lineage

Cell Proliferation

Cells, Cultured

Oligodendroglia

Receptor, Platelet-Derived Growth Factor alpha

Spinal Cord

Stem Cells

Authors

Marques, Sueli

van Bruggen, David

Vanichkina, Darya Pavlovna

Floriddia, Elisa Mariagrazia

Munguba, Hermany

Väremo, Leif

Giacomello, Stefania

Falcão, Ana Mendanha

Meijer, Mandy

Björklund, Åsa Kristina

Hjerling-Leffler, Jens

Taft, Ryan James

Castelo-Branco, Gonçalo

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