Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.

IF: 7.294

Cited by: 13

Abstract

The molecular mechanism underpinning the specification of the ectoderm, a transient germ-layer tissue, during mouse gastrulation was examined here in a stem cell-based model. We captured a self-renewing cell population with enhanced ectoderm potency from mouse epiblast stem cells (EpiSCs) by suppressing Nodal signaling activity. The transcriptome of the Nodal-inhibited EpiSCs resembles that of the anterior epiblast of embryonic day (E)7.0 and E7.5 mouse embryo, which is accompanied by chromatin modifications that reflect the priming of ectoderm lineage-related genes for expression. Nodal-inhibited EpiSCs show enhanced ectoderm differentiation in vitro and contribute to the neuroectoderm and the surface ectoderm in postimplantation chimeras but lose the propensity for mesendoderm differentiation in vitro and in chimeras. Our findings show that specification of the ectoderm progenitors is enhanced by the repression of Nodal signaling activity, and the ectoderm-like stem cells provide an experimental model to investigate the molecular characters of the epiblast-derived ectoderm.

Keywords

Gene Expression

PROCEDURE

ectoderm propensity

epiblast stem cells

histone modification

nodal signaling

transcriptome

MeSH terms

Animals

Biomarkers

Cell Differentiation

Cell Lineage

Cells, Cultured

Ectoderm

Embryonic Development

Epigenesis, Genetic

Fluorescent Antibody Technique

Gene Expression Profiling

Gene Expression Regulation, Developmental

Germ Layers

Mice

Nodal Protein

Signal Transduction

Wnt Signaling Pathway

Authors

Liu, Chang

Wang, Ran

He, Zhisong

Osteil, Pierre

Wilkie, Emilie

Yang, Xianfa

Chen, Jun

Cui, Guizhong

Guo, Wenke

Chen, Yingying

Peng, Guangdun

Tam, Patrick P L

Jing, Naihe

Recommend literature

1. Mouse gastrulation: Attributes of transcription factor regulatory network for epiblast patterning.

2. Distinct enhancer signatures in the mouse gastrula delineate progressive cell fate continuum during embryo development.

3. Molecular architecture of lineage allocation and tissue organization in early mouse embryo.

4. Spatial Transcriptome for the Molecular Annotation of Lineage Fates and Cell Identity in Mid-gastrula Mouse Embryo.

5. A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates.

Similar data

1. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells

2. Developmental equivalence of epiblast stem cells (EpiSCs)

3. Genome-wide comparison of EpiSC lines derived from fertilized (FT) and somatic cell nuclear transfer (NT) embryos

4. Transcript assembly and abundance estimation from RNA-Seq reveals thousands of new transcripts and switching among isoforms

5. Reorganization of enhancer patterns in transition from naïve to primed pluripotency (ChIP-seq)