Tracing the temporal-spatial transcriptome landscapes of the human fetal digestive tract using single-cell RNA-sequencing.
IF: 28.213
Cited by: 107


The development of the digestive tract is critical for proper food digestion and nutrient absorption. Here, we analyse the main organs of the digestive tract, including the oesophagus, stomach, small intestine and large intestine, from human embryos between 6 and 25 weeks of gestation as well as the large intestine from adults using single-cell RNA-seq analyses. In total, 5,227 individual cells are analysed and 40 cell types clearly identified. Their crucial biological features, including developmental processes, signalling pathways, cell cycle, nutrient digestion and absorption metabolism, and transcription factor networks, are systematically revealed. Moreover, the differentiation and maturation processes of the large intestine are thoroughly investigated by comparing the corresponding transcriptome profiles between embryonic and adult stages. Our work offers a rich resource for investigating the gene regulation networks of the human fetal digestive tract and adult large intestine at single-cell resolution.


Spatial reconstruction
Gene Expression

MeSH terms

Age Factors
Cell Differentiation
Cell Proliferation
Epithelial Cells
Gastrointestinal Tract
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genetic Markers
Gestational Age
Sequence Analysis, RNA
Single-Cell Analysis
Time Factors


Gao, Shuai
Yan, Liying
Wang, Rui
Li, Jingyun
Yong, Jun
Zhou, Xin
Wei, Yuan
Wu, Xinglong
Wang, Xiaoye
Fan, Xiaoying
Yan, Jie
Zhi, Xu
Gao, Yun
Guo, Hongshan
Jin, Xiao
Wang, Wendong
Mao, Yunuo
Wang, Fengchao
Wen, Lu
Fu, Wei
Ge, Hao
Qiao, Jie
Tang, Fuchou

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