Identification and expression pattern analysis of BoMYB51 involved in indolic glucosinolate biosynthesis from broccoli (Brassica oleracea var. italica).

PMID:29753738

IF: 3.322

Cited by: 8

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Abstract

Glucosinolates are a class of amino acid-derived specialized metabolites characteristic of the Brassicales order. Trp derived indolic glucosinolates are essential for the effective plant defense responses to a wide range of pathogens and herbivores. In Arabidopsis, MYB51 is the key transcription factor positively regulates indolic glucosinolate production by activating certain biosynthetic genes. In this study, we report the isolation and identification of a MYB51 from broccoli designated as BoMYB51. Overexpression of BoMYB51 in Arabidopsis increased indolic glucosinolate production by upregulating biosynthetic genes and resulted in enhanced flagellin22 (Flg22) induced callose deposition. The spatial expression pattern and responsive expression of BoMYB51 to several hormones and stress treatments were investigated by expressing the β-glucuronidase (GUS) reporter gene driven by BoMYB51 promotor in Arabidopsis and quantitative real-time PCR analysis in broccoli. Our study provides information on molecular characteristics of BoMYB51 and possible physiological process BoMYB51 may involve.

Keywords

Spatial Gene Expression

BoMYB51

Broccoli

Callose deposition

Indolic glucosinolates

Spatial expression pattern

MeSH terms

Arabidopsis

Biosynthetic Pathways

Brassica

Cloning, Molecular

Gene Expression Regulation, Plant

Genes, Plant

Glucosinolates

Plant Proteins

Transcription Factors

Authors

Yu, Qingyue

Hao, Guodong

Zhou, Jianxin

Wang, Jingying

Evivie, Ejiroghene Ruona

Li, Jing

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