The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution.
IF: 63.714
Cited by: 402


Time series of single-cell transcriptome measurements can reveal dynamic features of cell differentiation pathways. From measurements of whole frog embryos spanning zygotic genome activation through early organogenesis, we derived a detailed catalog of cell states in vertebrate development and a map of differentiation across all lineages over time. The inferred map recapitulates most if not all developmental relationships and associates new regulators and marker genes with each cell state. We find that many embryonic cell states appear earlier than previously appreciated. We also assess conflicting models of neural crest development. Incorporating a matched time series of zebrafish development from a companion paper, we reveal conserved and divergent features of vertebrate early developmental gene expression programs.


Spatial reconstruction
Gene Expression

MeSH terms

Cell Differentiation
Embryonic Development
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genetic Variation
Neural Crest
Pluripotent Stem Cells
Sequence Analysis, RNA
Single-Cell Analysis
Transcription Factors


Briggs, James A
Weinreb, Caleb
Wagner, Daniel E
Megason, Sean
Peshkin, Leonid
Kirschner, Marc W
Klein, Allon M

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