A hybridization-chain-reaction-based method for amplifying immunosignals.
Abstract
Immunosignal hybridization chain reaction (isHCR) combines antibody-antigen interactions with hybridization chain reaction (HCR) technology, which results in amplification of immunofluorescence signals by up to two to three orders of magnitude with low background. isHCR's highly modular and easily adaptable design enables the technique to be applied broadly, and we further optimized its use in multiplexed imaging and in state-of-the-art tissue expansion and clearing techniques.
Keywords
isHCR
Gene Expression
MeSH terms
Animals
Antibodies
Antigens
Behavior, Animal
Brain
Gene Expression Regulation
HEK293 Cells
Humans
Immunohistochemistry
Mice
Nucleic Acid Amplification Techniques
Nucleic Acid Hybridization
Protein Binding
Authors
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