Rapid Loss of RNA Detection by In Situ Hybridization in Stored Tissue Blocks and Preservation by Cold Storage of Unstained Slides.
PMID:29106457
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IF: 5.400
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Cited by: 47
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Abstract

Recent commercialization of methods for in situ hybridization using Z-pair probe/branched DNA amplification has led to increasing adoption of this technology for interrogating RNA expression in formalin-fixed, paraffin-embedded (FFPE) tissues. Current practice for FFPE block storage is to maintain them at room temperature, often for many years. To examine the effects of block storage time on FFPE tissues using a number of RNA in situ probes with the Advanced Cellular Diagnostic's RNAscope assay. We report marked reductions in signals after 5 years and significant reductions often after 1 year. Furthermore, storing unstained slides cut from recent cases (<1 year old) at -20°C can preserve hybridization signals significantly better than storing the blocks at room temperature and cutting the slides fresh when needed. We submit that the standard practice of storing FFPE tissue blocks at room temperature should be reevaluated to better preserve RNA for in situ hybridization.

Keywords

RNAscope
Gene Expression
Branched DNA amplification
Formalin-fixed
RNA in situ hybridization
paraffin-embedded tissues

MeSH terms

Animals
Antigens, Neoplasm
Cold Temperature
Cryopreservation
Humans
In Situ Hybridization
Male
Mice
Paraffin Embedding
Prostatic Neoplasms
RNA
Tissue Array Analysis
Tissue Fixation

Authors

Baena-Del Valle, Javier A
Zheng, Qizhi
Hicks, Jessica L
Fedor, Helen
Trock, Bruce J
Morrissey, Colm
Corey, Eva
Cornish, Toby C
Sfanos, Karen S
De Marzo, Angelo M

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