Functional Characterization of Xenopus Thyroid Hormone Transporters mct8 and oatp1c1.
IF: 5.051
Cited by: 7


Xenopus is an excellent model for studying thyroid hormone signaling as it undergoes thyroid hormone-dependent metamorphosis. Despite the fact that receptors and deiodinases have been described in Xenopus, membrane transporters for these hormones are yet to be characterized. We cloned Xenopus monocarboxylate transporter 8 (mct8) and organic anion-transporting polypeptide 1C1 (oatpc1c1), focusing on these two transporters given their importance for vertebrate brain development. Protein alignment and bootstrap analysis showed that Xenopus mct8 and oatp1c1 are closer to their mammalian orthologs than their teleost counterparts. We functionally characterized the two transporters using a radiolabeled hormones in vitro uptake assay in COS-1 cells. Xenopus mct8 was found to actively transport both T3 and T4 bidirectionally. As to the thyroid precursor molecules, diiodotyrosine (DIT) and monoiodotyrosine (MIT), both human and Xenopus mct8, showed active efflux, but no influx. Again similar to humans, Xenopus oatp1c1 transported T4 but not T3, MIT, or DIT. We used reverse transcription quantitative polymerase chain reaction and in situ hybridization to characterize the temporal and spatial expression of mct8 and oatp1c1 in Xenopus. Specific expression of the transporter was observed in the brain, with increasingly strong expression as development progressed. In conclusion, these results show that Xenopus thyroid hormone transporters are functional and display marked spatiotemporal expression patterns. These features make them interesting targets to elucidate their roles in determining thyroid hormone availability during embryonic development.


In situ hybridisation

MeSH terms

Amino Acid Sequence
Body Patterning
Cloning, Molecular
Crosses, Genetic
Gene Expression Regulation
Monocarboxylic Acid Transporters
Organic Anion Transporters
Thyroid Hormones


Mughal, Bilal B
Leemans, Michelle
Lima de Souza, Elaine C
le Mevel, Sébastien
Spirhanzlova, Petra
Visser, Theo J
Fini, Jean-Baptiste
Demeneix, Barbara A

Recommend literature