Laser capture microscopy coupled with Smart-seq2 for precise spatial transcriptomic profiling.
IF: 17.694
Cited by: 166


Laser capture microscopy (LCM) coupled with global transcriptome profiling could enable precise analyses of cell populations without the need for tissue dissociation, but has so far required relatively large numbers of cells. Here we report a robust and highly efficient strategy for LCM coupled with full-length mRNA-sequencing (LCM-seq) developed for single-cell transcriptomics. Fixed cells are subjected to direct lysis without RNA extraction, which both simplifies the experimental procedures as well as lowers technical noise. We apply LCM-seq on neurons isolated from mouse tissues, human post-mortem tissues, and illustrate its utility down to single captured cells. Importantly, we demonstrate that LCM-seq can provide biological insight on highly similar neuronal populations, including motor neurons isolated from different levels of the mouse spinal cord, as well as human midbrain dopamine neurons of the substantia nigra compacta and the ventral tegmental area.


Spatial Transcriptomics

MeSH terms

Dopaminergic Neurons
Gene Expression
Gene Expression Profiling
Laser Capture Microdissection
Models, Animal
Motor Neurons
Mouse Embryonic Stem Cells
Pars Compacta
Poly A
RNA, Messenger
Sequence Analysis, RNA
Spinal Cord
Ventral Tegmental Area


Nichterwitz, Susanne
Chen, Geng
Aguila Benitez, Julio
Yilmaz, Marlene
Storvall, Helena
Cao, Ming
Sandberg, Rickard
Deng, Qiaolin
Hedlund, Eva

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