Seeing is believing: new methods for in situ single-cell transcriptomics.
New methods employ RNA-seq to study single cells within complex tissues by in situ sequencing or mRNA capture from single photoactivated cells.
Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Sequence Analysis, RNA
1. RNA Tomography for Spatially Resolved Transcriptomics (Tomo-Seq).
2. Topographical transcriptome mapping of the mouse medial ganglionic eminence by spatially resolved RNA-seq.
3. Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue.
4. RNA sequencing: new technologies and applications in cancer research.
5. Ultra-high-throughput single-cell RNA sequencing and perturbation screening with combinatorial fluidic indexing.
1. Transcriptome In Vivo Analysis (TIVA) of spatially defined single cells in intact live mouse and human brain tissue
2. Single-cell transcriptomics reveals widespread heterogeneity in gene regulation and RNA processing in stimulated immune cells
3. Characterization of the single-cell (ES and MEF) transcriptional landscape by highly multiplex RNA-Seq
4. RNA-seq of laser-capture microdissected 50x50x50 um tissue cubes from the medial ganglionic eminence of wild-type and GFRa1 mutant mice
5. Comparative gene expression profiling of human PGP1 iPS, lymphocyte, donor fibroblasts