Temporospatial expression of fibulin-1 after acute spinal cord injury in rats.

IF: 2.040

Cited by: 1

Abstract

Fibulin-1 is a matricellular protein that plays important roles in motility inhibition in a variety of cells and blocks the proliferation of cultured neural stem cells. The biological function of fibulin-1 in the spinal cord has not been fully elucidated. To clarify the expressions and possible functions of fibulin-1 in spinal cord injury (SCI), we performed an acute spinal cord contusion injury model in adult rats. Our work studied the temporospatial expression patterns of fibulin-1. Western blot analysis revealed that fibulin-1 levels significantly increased 5 days after spinal cord contusion. Immunohistochemistry confirmed an increased number of fibulin-1 immunopositive cells about 2 mm from the lesion site. Moreover, double immunofluorescence labeling suggested that these changes were especially prominent in neurons and microglia. These findings suggest that fibulin-1 may be involved in neuronal apoptosis and microglial activation after SCI.

Keywords

Spatial Gene Expression

Fibulin-1

Glial proliferation

Neuron apoptosis

Rat

Spinal cord injury

MeSH terms

Animals

Apoptosis

Calcium-Binding Proteins

Male

Organ Specificity

Rats

Rats, Sprague-Dawley

Spinal Cord

Spinal Cord Injuries

Authors

Xu, Guanhua

Cui, Ying

Wang, Lingling

Zhang, Jinlong

Shen, Aiguo

Li, Weidong

Bao, Guofeng

Sun, Yuyu

Cui, Zhiming

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