XBP1S, a BMP2-inducible transcription factor, accelerates endochondral bone growth by activating GEP growth factor.
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IF: 5.295
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Cited by: 9
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Abstract

We previously reported that transcription factor XBP1S binds to RUNX2 and enhances chondrocyte hypertrophy through acting as a cofactor of RUNX2. Herein, we report that XBP1S is a key downstream molecule of BMP2 and is required for BMP2-mediated chondrocyte differentiation. XBP1S is up-regulated during chondrocyte differentiation and demonstrates the temporal and spatial expression pattern during skeletal development. XBP1S stimulates chondrocyte differentiation from mesenchymal stem cells in vitro and endochondral ossification ex vivo. In addition, XBP1S activates granulin-epithelin precursor (GEP), a growth factor known to stimulate chondrogenesis, and endogenous GEP is required, at least in part, for XBP1S-stimulated chondrocyte hypertrophy, mineralization and endochondral bone formation. Furthermore, XBP1S enhances GEP-stimulated chondrogenesis and endochondral bone formation. Collectively, these findings demonstrate that XBP1S, a BMP2-inducible transcription factor, positively regulates endochondral bone formation by activating GEP chondrogenic growth factor.

Keywords

BMP2
GEP
X-box binding protein 1 spliced (XBP1S)
chondrogenesis
unfolded protein response

MeSH terms

Animals
Blotting, Western
Bone Morphogenetic Protein 2
Cell Differentiation
Cells, Cultured
Chondrogenesis
Chromatin Immunoprecipitation
DNA-Binding Proteins
Female
Gene Expression Regulation, Developmental
Immunoenzyme Techniques
Intercellular Signaling Peptides and Proteins
Mesenchymal Stem Cells
Mice
Mice, Inbred C3H
Mice, Knockout
Osteogenesis
Progranulins
RNA, Messenger
Real-Time Polymerase Chain Reaction
Regulatory Factor X Transcription Factors
Reverse Transcriptase Polymerase Chain Reaction
Transcription Factors
X-Box Binding Protein 1

Authors

Guo, Feng-Jin
Xiong, Zhangyuan
Han, Xiaofeng
Liu, Chuanju
Liu, Yanna
Jiang, Rong
Zhang, Peng

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