Highly multiplexed imaging of tumor tissues with subcellular resolution by mass cytometry.
IF: 47.990
Cited by: 1,241


Mass cytometry enables high-dimensional, single-cell analysis of cell type and state. In mass cytometry, rare earth metals are used as reporters on antibodies. Analysis of metal abundances using the mass cytometer allows determination of marker expression in individual cells. Mass cytometry has previously been applied only to cell suspensions. To gain spatial information, we have coupled immunohistochemical and immunocytochemical methods with high-resolution laser ablation to CyTOF mass cytometry. This approach enables the simultaneous imaging of 32 proteins and protein modifications at subcellular resolution; with the availability of additional isotopes, measurement of over 100 markers will be possible. We applied imaging mass cytometry to human breast cancer samples, allowing delineation of cell subpopulations and cell-cell interactions and highlighting tumor heterogeneity. Imaging mass cytometry complements existing imaging approaches. It will enable basic studies of tissue heterogeneity and function and support the transition of medicine toward individualized molecularly targeted diagnosis and therapies.


Gene Expression

MeSH terms

Breast Neoplasms
Cell Line
Epithelial Cells
Gene Expression Regulation, Neoplastic
Image Cytometry
Neoplasm Proteins


Giesen, Charlotte
Wang, Hao A O
Schapiro, Denis
Zivanovic, Nevena
Jacobs, Andrea
Hattendorf, Bodo
Schüffler, Peter J
Grolimund, Daniel
Buhmann, Joachim M
Brandt, Simone
Varga, Zsuzsanna
Wild, Peter J
Günther, Detlef
Bodenmiller, Bernd

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