Multiplexed ion beam imaging of human breast tumors.
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IF: 87.241
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Cited by: 773
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Abstract

Immunohistochemistry (IHC) is a tool for visualizing protein expression that is employed as part of the diagnostic workup for the majority of solid tissue malignancies. Existing IHC methods use antibodies tagged with fluorophores or enzyme reporters that generate colored pigments. Because these reporters exhibit spectral and spatial overlap when used simultaneously, multiplexed IHC is not routinely used in clinical settings. We have developed a method that uses secondary ion mass spectrometry to image antibodies tagged with isotopically pure elemental metal reporters. Multiplexed ion beam imaging (MIBI) is capable of analyzing up to 100 targets simultaneously over a five-log dynamic range. Here, we used MIBI to analyze formalin-fixed, paraffin-embedded human breast tumor tissue sections stained with ten labels simultaneously. The resulting data suggest that MIBI can provide new insights into disease pathogenesis that will be valuable for basic research, drug discovery and clinical diagnostics.

Keywords

SIMS
MIBI
Gene Expression

MeSH terms

Biomarkers, Tumor
Breast Neoplasms
Estrogen Receptor alpha
Female
Humans
Immunohistochemistry
Mass Spectrometry
Receptor, ErbB-2
Receptors, Progesterone

Authors

Angelo, Michael
Bendall, Sean C
Finck, Rachel
Hale, Matthew B
Hitzman, Chuck
Borowsky, Alexander D
Levenson, Richard M
Lowe, John B
Liu, Scot D
Zhao, Shuchun
Natkunam, Yasodha
Nolan, Garry P

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