In situ sequencing for RNA analysis in preserved tissue and cells.
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IF: 47.990
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Cited by: 428
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Abstract

Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.

Keywords

Gene Expression
ISS

MeSH terms

Actins
Breast Neoplasms
Female
Gene Expression Profiling
Humans
Point Mutation
Proto-Oncogene Proteins
Proto-Oncogene Proteins p21(ras)
RNA, Messenger
Receptor, ErbB-2
Sequence Analysis, RNA
Tissue Preservation
ras Proteins

Authors

Ke, Rongqin
Mignardi, Marco
Pacureanu, Alexandra
Svedlund, Jessica
Botling, Johan
Wählby, Carolina
Nilsson, Mats

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