In situ sequencing for RNA analysis in preserved tissue and cells.
Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.
Gene Expression Profiling
Proto-Oncogene Proteins p21(ras)
Sequence Analysis, RNA
1. Highly multiplexed subcellular RNA sequencing in situ.
2. Single-cell in situ RNA profiling by sequential hybridization.
3. RNA imaging. Spatially resolved, highly multiplexed RNA profiling in single cells.
4. Three-dimensional intact-tissue sequencing of single-cell transcriptional states.
5. In Situ Transcription Profiling of Single Cells Reveals Spatial Organization of Cells in the Mouse Hippocampus.
1. Alternative Isoform Regulation in Human Tissue Transcriptomes
2. Comparative gene expression profiling of human PGP1 iPS, lymphocyte, donor fibroblasts
3. Distinct oligodendrocyte populations have spatial preference and different responses to spinal cord injury
4. High-density spatial transcriptomics arrays for in situ tissue profiling
5. Molecular Atlas of the Adult Mouse Brain