miR-370 is stage-specifically expressed during mouse embryonic development and regulates Dnmt3a.
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that participate in a large variety of biological processes. In this paper, the spatiotemporal expression pattern of miR-370 was characterized during mouse embryonic development, and was found to be stage- and tissue-specifically expressed. In addition, through luciferase reporter assays and western blot analyses, DNA methyltransferase 3A (Dnmt3a) was identified as a directly regulated target of miR-370. Altogether, our results indicate that miR-370 may play important roles in the morphogenesis of diverse organs, especially brain and adrenal glands, by mediating Dnmt3a expression during mouse development.
Keywords
Temporal Gene Expression
MeSH terms
Adrenal Glands
Animals
Base Sequence
Brain
DNA (Cytosine-5-)-Methyltransferases
DNA Methyltransferase 3A
Embryo, Mammalian
Female
Gene Expression Regulation, Developmental
Genes, Reporter
Luciferases
Mice
Mice, Inbred ICR
MicroRNAs
Molecular Sequence Data
Organ Specificity
Signal Transduction
Time Factors
Authors
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