Use of in situ hybridization to examine gene expression in the embryonic, neonatal, and adult urogenital system.


Studies into the molecular basis of morphogenesis frequently begin with investigations into gene expression across time and cell type in that organ. One of the most anatomically informative approaches to such studies is the use of in situ hybridization, either of intact or histologically sectioned tissues. Here, we describe the optimization of this approach for use in the temporal and spatial analysis of gene expression in the urogenital system, from embryonic development to the postnatal period. The methods described are applicable for high throughput analysis of large gene sets. As such, ISH has become a powerful technique for gene expression profiling and is valuable for the validation of profiling analyses performed using other approaches such as microarrays.


Spatial Gene Expression
Spatial Anatomic

MeSH terms

Gene Expression Profiling
Gene Expression Regulation, Developmental
In Situ Hybridization
RNA, Messenger
Urogenital System


Rumballe, Bree A
Chiu, Han Sheng
Georgas, Kylie M
Little, Melissa H

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