Expression of macro non-coding RNAs Meg8 and Irm in mouse embryonic development.
IF: 2.147
Cited by: 19


Non-coding RNAs (ncRNAs) Meg8 and Irm were previously identified as alternatively splicing isoforms of Rian gene. Ascertaining ncRNAs spatiotemporal expression patterns is crucial for understanding the physiological roles of ncRNAs during tissue and organ development. In this study in mouse embryos, we focused on the developmental regulation expression of imprinted macro ncRNAs, Meg8 and Irm by using in situ hybridization and quantitative real-time RT-PCR (QRT-PCR). The in situ hybridization results showed that Meg8 and Irm were expressed in the developing brain at embryonic day 10.5 (E10.5) and E11.5, while Irm expression signals were strikingly detected in the somite, where Meg8 expression signals were undetectable. By E15.5, they were expressed in brain, tongue, liver, lung and neuroendocrine tissues, while Irm displayed more restricted expression in tongue and skeletal muscle than Meg8. Furthermore, quantitative analysis confirmed that they were highly expressed in tongue and brain at E12.5, E15.5 and E18.5. These results indicated that Meg8 and Irm might be coordinately expressed and functionally correlated in diverse of organs. Notably, Irm was more closely associated with morphogenesis of skeletal muscle in contrast to Meg8 during embryonic development.


Temporal Gene Expression

MeSH terms

Alternative Splicing
Embryonic Development
Gene Expression Regulation, Developmental
Genomic Imprinting
In Situ Hybridization
Mice, Inbred C57BL
Muscle, Skeletal
Nuclear Proteins
Organ Specificity
RNA, Untranslated
Real-Time Polymerase Chain Reaction


Gu, Tiantian
He, Hongjuan
Han, Zhengbin
Zeng, Tiebo
Huang, Zhijun
Liu, Qi
Gu, Ning
Chen, Yan
Sugimoto, Kenkichi
Jiang, Huijie
Wu, Qiong

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