Validating transcripts with probes and imaging technology.

IF: 47.990

Cited by: 177

Abstract

High-throughput gene expression screens provide a quantitative picture of the average expression signature of biological samples. However, the analysis of spatial gene expression patterns with single-cell resolution requires quantitative in situ measurement techniques. Here we describe recent technological advances in RNA fluorescence in situ hybridization (FISH) techniques that facilitate detection of individual fluorescently labeled mRNA molecules of practically any endogenous gene. These methods, which are based on advances in probe design, imaging technology and image processing, enable the absolute measurement of transcript abundance in individual cells with single-molecule resolution.

Keywords

LCM-seq

Spatial Gene Expression

MeSH terms

Animals

DNA Barcoding, Taxonomic

Data Interpretation, Statistical

Fluorescent Dyes

Gene Expression Profiling

Humans

In Situ Hybridization, Fluorescence

RNA Probes

RNA, Messenger

Single-Cell Analysis

Validation Studies as Topic

Authors

Itzkovitz, Shalev

van Oudenaarden, Alexander

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