In situ detection and genotyping of individual mRNA molecules.
Abstract
Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
Keywords
Gene Expression
MeSH terms
Actins
Animals
Cell Line, Tumor
Cells, Cultured
Humans
Mice
Nucleic Acid Amplification Techniques
Point Mutation
RNA, Messenger
Receptor, ErbB-2
Transcription, Genetic
Authors
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