Differentiation driven changes in the dynamic organization of Basal transcription initiation.
IF: 9.593
Cited by: 46


Studies based on cell-free systems and on in vitro-cultured living cells support the concept that many cellular processes, such as transcription initiation, are highly dynamic: individual proteins stochastically bind to their substrates and disassemble after reaction completion. This dynamic nature allows quick adaptation of transcription to changing conditions. However, it is unknown to what extent this dynamic transcription organization holds for postmitotic cells embedded in mammalian tissue. To allow analysis of transcription initiation dynamics directly into living mammalian tissues, we created a knock-in mouse model expressing fluorescently tagged TFIIH. Surprisingly and in contrast to what has been observed in cultured and proliferating cells, postmitotic murine cells embedded in their tissue exhibit a strong and long-lasting transcription-dependent immobilization of TFIIH. This immobilization is both differentiation driven and development dependent. Furthermore, although very statically bound, TFIIH can be remobilized to respond to new transcriptional needs. This divergent spatiotemporal transcriptional organization in different cells of the soma revisits the generally accepted highly dynamic concept of the kinetic framework of transcription and shows how basic processes, such as transcription, can be organized in a fundamentally different fashion in intact organisms as previously deduced from in vitro studies.

MeSH terms

Bacterial Proteins
Cell Differentiation
Cells, Cultured
Cerebral Cortex
Chromatin Immunoprecipitation
Embryonic Stem Cells
Fluorescence Recovery After Photobleaching
Gene Expression Regulation, Developmental
Luminescent Proteins
Mice, Inbred C57BL
Organ Culture Techniques
Recombinant Fusion Proteins
Transcription Factor TFIIH
Transcription, Genetic


Giglia-Mari, Giuseppina
Theil, Arjan F
Mari, Pierre-Olivier
Mourgues, Sophie
Nonnekens, Julie
Andrieux, Lise O
de Wit, Jan
Miquel, Catherine
Wijgers, Nils
Maas, Alex
Fousteri, Maria
Hoeijmakers, Jan H J
Vermeulen, Wim

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